A strategy for characterization of persistent heteroduplex DNA in higher plants.
Identifieur interne : 002388 ( Main/Exploration ); précédent : 002387; suivant : 002389A strategy for characterization of persistent heteroduplex DNA in higher plants.
Auteurs : Chun-Bo Dong [République populaire de Chine] ; Jian-Feng Mao ; Yu-Jing Suo ; Le Shi ; Jun Wang ; Ping-Dong Zhang ; Xiang-Yang KangSource :
- The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Plant.
- genetics : Plants.
- Homologous Recombination, Nucleic Acid Heteroduplexes.
Abstract
Heteroduplex DNA (hDNA) generated during homologous recombination (HR) is an important component that shapes genetic diversity in sexually reproducing organisms. However, studies of this process in higher plants are limited. This is because hDNAs are difficult to capture in higher plants as their reproductive developmental model only produces normal gametes and does not preserve the mitotic products of the post-meiotic segregation (PMS) process which is crucial for studying hDNAs. In this study, using the model system for tree and woody perennial plant biology (Populus), we propose a strategy for characterizing hDNAs in higher plants. We captured hDNAs by constructing triploid hybrids originating from a cross between unreduced 2n eggs (containing hDNA information as a result of inhibition chromosome segregation at the PMS stage) with normal male gametes. These triploid hybrids allowed us to detect the frequency and location of persistent hDNAs resulting from HR at the molecular level. We found that the frequency of persistent hDNAs, which ranged from 5.3 to 76.6%, was related to locations of the simple sequence repeat markers at the chromosomes, such as the locus-centromere distance, the surrounding DNA sequence and epigenetic information, and the richness of protein-coding transcripts at these loci. In summary, this study provides a method for characterizing persistent hDNAs in higher plants. When high-throughput sequencing techniques can be incorporated, genome-wide persistent hDNA assays for higher plants can be easily carried out using the strategy presented in this study.
DOI: 10.1111/tpj.12631
PubMed: 25073546
Affiliations:
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Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083</wicri:regionArea><wicri:noRegion>100083</wicri:noRegion></affiliation></author><author><name sortKey="Mao, Jian Feng" sort="Mao, Jian Feng" uniqKey="Mao J" first="Jian-Feng" last="Mao">Jian-Feng Mao</name></author><author><name sortKey="Suo, Yu Jing" sort="Suo, Yu Jing" uniqKey="Suo Y" first="Yu-Jing" last="Suo">Yu-Jing Suo</name></author><author><name sortKey="Shi, Le" sort="Shi, Le" uniqKey="Shi L" first="Le" last="Shi">Le Shi</name></author><author><name sortKey="Wang, Jun" sort="Wang, Jun" uniqKey="Wang J" first="Jun" last="Wang">Jun Wang</name></author><author><name sortKey="Zhang, Ping Dong" sort="Zhang, Ping Dong" uniqKey="Zhang P" first="Ping-Dong" last="Zhang">Ping-Dong Zhang</name></author><author><name sortKey="Kang, Xiang Yang" sort="Kang, Xiang Yang" uniqKey="Kang X" first="Xiang-Yang" last="Kang">Xiang-Yang Kang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25073546</idno><idno type="pmid">25073546</idno><idno type="doi">10.1111/tpj.12631</idno><idno type="wicri:Area/Main/Corpus">002071</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002071</idno><idno type="wicri:Area/Main/Curation">002071</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002071</idno><idno type="wicri:Area/Main/Exploration">002071</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A strategy for characterization of persistent heteroduplex DNA in higher plants.</title><author><name sortKey="Dong, Chun Bo" sort="Dong, Chun Bo" uniqKey="Dong C" first="Chun-Bo" last="Dong">Chun-Bo Dong</name><affiliation wicri:level="1"><nlm:affiliation>National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, China; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, China; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083</wicri:regionArea><wicri:noRegion>100083</wicri:noRegion></affiliation></author><author><name sortKey="Mao, Jian Feng" sort="Mao, Jian Feng" uniqKey="Mao J" first="Jian-Feng" last="Mao">Jian-Feng Mao</name></author><author><name sortKey="Suo, Yu Jing" sort="Suo, Yu Jing" uniqKey="Suo Y" first="Yu-Jing" last="Suo">Yu-Jing Suo</name></author><author><name sortKey="Shi, Le" sort="Shi, Le" uniqKey="Shi L" first="Le" last="Shi">Le Shi</name></author><author><name sortKey="Wang, Jun" sort="Wang, Jun" uniqKey="Wang J" first="Jun" last="Wang">Jun Wang</name></author><author><name sortKey="Zhang, Ping Dong" sort="Zhang, Ping Dong" uniqKey="Zhang P" first="Ping-Dong" last="Zhang">Ping-Dong Zhang</name></author><author><name sortKey="Kang, Xiang Yang" sort="Kang, Xiang Yang" uniqKey="Kang X" first="Xiang-Yang" last="Kang">Xiang-Yang Kang</name></author></analytic><series><title level="j">The Plant journal : for cell and molecular biology</title><idno type="eISSN">1365-313X</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Plant (genetics)</term><term>Homologous Recombination (MeSH)</term><term>Nucleic Acid Heteroduplexes (MeSH)</term><term>Plants (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term><term>Hétéroduplexes d'acides nucléiques (MeSH)</term><term>Plantes (génétique)</term><term>Recombinaison homologue (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des plantes</term><term>Plantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Homologous Recombination</term><term>Nucleic Acid Heteroduplexes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Hétéroduplexes d'acides nucléiques</term><term>Recombinaison homologue</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Heteroduplex DNA (hDNA) generated during homologous recombination (HR) is an important component that shapes genetic diversity in sexually reproducing organisms. However, studies of this process in higher plants are limited. This is because hDNAs are difficult to capture in higher plants as their reproductive developmental model only produces normal gametes and does not preserve the mitotic products of the post-meiotic segregation (PMS) process which is crucial for studying hDNAs. In this study, using the model system for tree and woody perennial plant biology (Populus), we propose a strategy for characterizing hDNAs in higher plants. We captured hDNAs by constructing triploid hybrids originating from a cross between unreduced 2n eggs (containing hDNA information as a result of inhibition chromosome segregation at the PMS stage) with normal male gametes. These triploid hybrids allowed us to detect the frequency and location of persistent hDNAs resulting from HR at the molecular level. We found that the frequency of persistent hDNAs, which ranged from 5.3 to 76.6%, was related to locations of the simple sequence repeat markers at the chromosomes, such as the locus-centromere distance, the surrounding DNA sequence and epigenetic information, and the richness of protein-coding transcripts at these loci. In summary, this study provides a method for characterizing persistent hDNAs in higher plants. When high-throughput sequencing techniques can be incorporated, genome-wide persistent hDNA assays for higher plants can be easily carried out using the strategy presented in this study. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25073546</PMID><DateCompleted><Year>2015</Year><Month>11</Month><Day>17</Day></DateCompleted><DateRevised><Year>2014</Year><Month>10</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-313X</ISSN><JournalIssue CitedMedium="Internet"><Volume>80</Volume><Issue>2</Issue><PubDate><Year>2014</Year><Month>Oct</Month></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>A strategy for characterization of persistent heteroduplex DNA in higher plants.</ArticleTitle><Pagination><MedlinePgn>282-91</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/tpj.12631</ELocationID><Abstract><AbstractText>Heteroduplex DNA (hDNA) generated during homologous recombination (HR) is an important component that shapes genetic diversity in sexually reproducing organisms. However, studies of this process in higher plants are limited. This is because hDNAs are difficult to capture in higher plants as their reproductive developmental model only produces normal gametes and does not preserve the mitotic products of the post-meiotic segregation (PMS) process which is crucial for studying hDNAs. In this study, using the model system for tree and woody perennial plant biology (Populus), we propose a strategy for characterizing hDNAs in higher plants. We captured hDNAs by constructing triploid hybrids originating from a cross between unreduced 2n eggs (containing hDNA information as a result of inhibition chromosome segregation at the PMS stage) with normal male gametes. These triploid hybrids allowed us to detect the frequency and location of persistent hDNAs resulting from HR at the molecular level. We found that the frequency of persistent hDNAs, which ranged from 5.3 to 76.6%, was related to locations of the simple sequence repeat markers at the chromosomes, such as the locus-centromere distance, the surrounding DNA sequence and epigenetic information, and the richness of protein-coding transcripts at these loci. In summary, this study provides a method for characterizing persistent hDNAs in higher plants. When high-throughput sequencing techniques can be incorporated, genome-wide persistent hDNA assays for higher plants can be easily carried out using the strategy presented in this study. </AbstractText><CopyrightInformation>© 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Chun-Bo</ForeName><Initials>CB</Initials><AffiliationInfo><Affiliation>National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, China; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mao</LastName><ForeName>Jian-Feng</ForeName><Initials>JF</Initials></Author><Author ValidYN="Y"><LastName>Suo</LastName><ForeName>Yu-Jing</ForeName><Initials>YJ</Initials></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Le</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jun</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ping-Dong</ForeName><Initials>PD</Initials></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Xiang-Yang</ForeName><Initials>XY</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>08</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant J</MedlineTA><NlmUniqueID>9207397</NlmUniqueID><ISSNLinking>0960-7412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009692">Nucleic Acid Heteroduplexes</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059765" MajorTopicYN="N">Homologous Recombination</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009692" MajorTopicYN="Y">Nucleic Acid Heteroduplexes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010944" MajorTopicYN="N">Plants</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Populus pseudo-simonii × Populus nigra ‘Zheyin3#’</Keyword><Keyword MajorTopicYN="N">Populus × beijingensis</Keyword><Keyword MajorTopicYN="N">heteroduplex DNA</Keyword><Keyword MajorTopicYN="N">post-meiotic segregation</Keyword><Keyword MajorTopicYN="N">triploid</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>08</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>07</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>07</Month><Day>25</Day></PubMedPubDate><PubMedPubDate 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last="Shi">Le Shi</name><name sortKey="Suo, Yu Jing" sort="Suo, Yu Jing" uniqKey="Suo Y" first="Yu-Jing" last="Suo">Yu-Jing Suo</name><name sortKey="Wang, Jun" sort="Wang, Jun" uniqKey="Wang J" first="Jun" last="Wang">Jun Wang</name><name sortKey="Zhang, Ping Dong" sort="Zhang, Ping Dong" uniqKey="Zhang P" first="Ping-Dong" last="Zhang">Ping-Dong Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Dong, Chun Bo" sort="Dong, Chun Bo" uniqKey="Dong C" first="Chun-Bo" last="Dong">Chun-Bo Dong</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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